Human R-Spondin 1 / RSPO1 Protein (His Tag) 11083-H08H from Sino Biological, Inc.

Product Specs
ItemHuman R-Spondin 1 / RSPO1 Protein (His Tag)
CompanySino Biological, Inc.
Price Supplier Page View Company Product Page
Catalog Number11083-H08H
Conjugate/TagC-His
Purity≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC
TypeRecombinant protein
Molecular WeightThe secreted recombinant human RSPO1 comprises 254 amino acids with a predicted molecular mass of 28.2 kDa. As a result of glycosylation, rhRSPO1 migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions
SpeciesHuman
Molecule NameRSPO1
Source/Expression SystemHEK293 Cells
NCBI Full Gene NameR-spondin 1
NCBI Gene AliasesCRISTIN3, RSPO
Activity1. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 µg/ml (100 µl/well) can bind human LIMPII with a linear range of 32-800 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 µg/ml (100 µl/well) can bind mouse CD36 with a linear range of 6.4-800 ng/ml. 3. Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.9 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt3a. 4.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human R-Spondin 1/RSPO1 Protein, His Tag (Cat. No. 11083-H08H) with an affinity constant of 0.983 µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested)
FormatLyophilized from sterile PBS, pH 7.4